Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Details-enriched characterization of mobile populations. Morphological info from photos can increase to the richness of flow cytometry info. For instance, the determine displays an normally traditional apoptosis assay utilizing Annexin V and PI, adding mobile imaging to characterize cells in each population to reveal morphologically distinctive capabilities. These insights couldn't have been attained from multiplex staining alone.
No matter whether you’re a novice or an expert, examine our means to progress your study and skills in flow cytometry.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The number of singlets available for Examination raises as soon as the user trains and employs a far more exact design.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating using a higher-velocity brightfield digicam for simultaneous significant throughput flow cytometry and superior resolution brightfield imaging.
*Pricing is indicative only and it is issue to vary. You should contact your Agilent consultant with any questions. The knowledge set forth herein is not an offer or possibly a dedication, both equally of which are matter to credit history approval, conditions and terms, and availability with Agilent. Agilent Fiscal Solutions is obtainable in picked countries.
Below, an experienced user has gated singlets confidently. Right after analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains in excess of 4% aggregates.
Optimizing gating approach. Even robust guide singlet gating is error-susceptible and continues to be a subjective conclusion level in Pretty much all flow cytometry assays. Imaging may be used to substantiate and regulate gates to include only single cells of desire.
Reliable picture top quality even at superior throughput Acoustic focusing along with a large-speed camera combine to graphic these T cells persistently at small or large flow premiums. Easily adjust concentrate and digital camera configurations to meet experimental specifications.
Early signs of this contamination had at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological options to find out appealing subpopulations that could not be obvious from flow cytometry information on your own.
Buyers can perspective event illustrations or photos in authentic-time to verify sample top quality, improve protocols, and document the morphology of each and every cell population determined for Assessment. Imaging seize also Rewards from get more info acoustic concentrating, which helps to posture the cells to ensure a pointy, centered impression is acquired.
Sound waves enable exact mobile alignment, causing exceptional knowledge excellent and ~10X more rapidly sample flow rates.
Application note: Automated impression Investigation cuts down user-to-user variability in flow cytometry gating procedures
Leverage the power of advanced know-how and innovation to speed up your discoveries. Believe in in the Attune Flow Cytometer family members to deliver the reliability, velocity, and accuracy that established your get the job done aside.
Adaptable payment system from Agilent Economic Remedies allow you to receive the significant instrumentation you'll need for fast project, or to grow your lab.
Gating on these prolonged parameters permits you to swiftly and properly recognize populations of fascination and confirm gating system with little if any handbook assessment. The Attune computer software features use of state-of-the-art machine Understanding analysis instruments in just an expertly developed user interface, making it exceptionally convenient to use.
As the mobile line experienced Earlier been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the images and were being ready to doc the microbial an infection at the very least five days earlier.
The Attune CytPix Flow Cytometer lets customers to visualise samples for the duration of acquisition, confirm the precision of gating methods, and help effects with reputable documentation for publication needs.
What seemed to be a homogeneous inhabitants is actually more numerous—an insight that should be regarded as when interpreting outcomes.